1Chukwuma Chinaza Adaobi, 2Cecil K. Dovia, 3Dr. Joseph Mawunyo Apeadido, and 1Felix Fiavor
1Catholic University College Fiapre, Sunyani Ghana
2Oti Regional Health Directorate
3Worawora Government hospital
Abstract: The current
investigation analyzed the effect of the combination of standardized hydroalcoholic
extracts of Asparagus racemosus (AR), Centella asiatica (CA), and Plumeria
rubra (PR) on glibenclamide (GB) administered in experimentally induced
diabetic nephropathy (DN) in Wistar rats. The study was conducted at the Heaven
Diagnostic Laboratory in Sunyani, and the DN was induced by alloxan monohydrate
(160 mg/kg i.p). Seven rats within 6 months (0.5) to 11 months (1.0) of age
were selected. The DN rats were given
the combination of AR, CA, and PR extracts as ACP (100, 200, and 400 mg/kg,
respectively) along with GB (5 mg/kg). Group 1-4 were cosntrol, diabetic
control, standard GB (10 mg/kg), GB (5 mg/kg), while groups 5-7 were given ACP
(100, 200 and 400 mg/kg) along with GB (5 mg/kg) once daily for 30 days. Blood
glucose, serum insulin, uric acid, blood urea nitrogen (BUN), creatinine,
superoxide dismutase (SOD), albumin, malondialdehyde (MDA), and kidney
histopathological examination was done in all the experimental animals. ACP
along with GB improved the derailed glycaemic status, renal biochemical, and
oxidative stress parameters in animals. Co-administration of ACP (400 mg/kg)
with GB (5 mg/kg) significantly (p<0.01) decreased BUN, creatinine, uric
acid, significantly increased (p<0.05) SOD, significantly (p<0.01)
decreased MDA, significantly increased (p<0.05) serum insulin when compared
with diabetic control. Histopathological observations corroborated with
biochemical parameters. Enhancement in the effectiveness of GB was observed
with ACP (400 mg/kg). Thus, ACP confers protection by improving renal,
oxidative parameters, and morphological abnormalities in the kidneys.
No comments:
Post a Comment