Friday, October 15, 2021

PROTECTIVE EFFECT OF THE COMBINATION OF HYDROALCOHOLIC EXTRACTS OF ASPARAGUS RACEMOSUS, CENTELLA ASIATICA, PLUMERIA RUBRA WITH GLIBENCLAMIDE IN EXPERIMENTALLY INDUCED DIABETIC NEPHROPATHY IN RATS

 1Chukwuma Chinaza Adaobi, 2Cecil K. Dovia, 3Dr. Joseph Mawunyo Apeadido, and 1Felix Fiavor

1Catholic University College Fiapre, Sunyani Ghana

2Oti Regional Health Directorate

3Worawora Government hospital

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Abstract: The current investigation analyzed the effect of the combination of standardized hydroalcoholic extracts of Asparagus racemosus (AR), Centella asiatica (CA), and Plumeria rubra (PR) on glibenclamide (GB) administered in experimentally induced diabetic nephropathy (DN) in Wistar rats. The study was conducted at the Heaven Diagnostic Laboratory in Sunyani, and the DN was induced by alloxan monohydrate (160 mg/kg i.p). Seven rats within 6 months (0.5) to 11 months (1.0) of age were selected.  The DN rats were given the combination of AR, CA, and PR extracts as ACP (100, 200, and 400 mg/kg, respectively) along with GB (5 mg/kg). Group 1-4 were cosntrol, diabetic control, standard GB (10 mg/kg), GB (5 mg/kg), while groups 5-7 were given ACP (100, 200 and 400 mg/kg) along with GB (5 mg/kg) once daily for 30 days. Blood glucose, serum insulin, uric acid, blood urea nitrogen (BUN), creatinine, superoxide dismutase (SOD), albumin, malondialdehyde (MDA), and kidney histopathological examination was done in all the experimental animals. ACP along with GB improved the derailed glycaemic status, renal biochemical, and oxidative stress parameters in animals. Co-administration of ACP (400 mg/kg) with GB (5 mg/kg) significantly (p<0.01) decreased BUN, creatinine, uric acid, significantly increased (p<0.05) SOD, significantly (p<0.01) decreased MDA, significantly increased (p<0.05) serum insulin when compared with diabetic control. Histopathological observations corroborated with biochemical parameters. Enhancement in the effectiveness of GB was observed with ACP (400 mg/kg). Thus, ACP confers protection by improving renal, oxidative parameters, and morphological abnormalities in the kidneys.

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Vol 6 Issue 01, October 2021

EMPIRICAL REVIEW OF VOCATIONAL CHOICE AMONG SENIOR HIGH SCHOOL STUDENTS 1 Samuel Dontoh, and 2 James Kwabena Odum 1 Department of Educat...